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. 2010 Feb 26;38(11):3546–3554. doi: 10.1093/nar/gkq097

Figure 3.

Figure 3.

Binding of MalE-GadE and (His)6-RcsB to the gadA promoter region. (A) EMSA with gadA promoter region-templates (from –101 to +24 relative to the +1start of transcription). No protein (lane 1), 10 µM MBP-GadE (lane 2), 0.1, 1 and 10 µM (His)6-RcsB plus 0.1 µM MBP-GadE (lanes 6–8) or plus 1 µM MBP-GadE (lanes 9–11), plus 10 µM MBP-GadE (lanes 12–14). (B) EMSA using protein from wt or rcs mutants with gadA promoter region templates. Proteins were purified from either a wild-type strain (lanes 3 and 4) or a ΔrcsA ΔrcsCDB ΔgadE strain (lanes 2, 5 and 6). No protein (lane 1), 1 µM (His)6-RcsB (lane 2), 3 µM MBP-GadE (lanes 3 and 5), 3 µM MBP-GadE and 1 µM (His)6-RcsB (lanes 4 and 6).