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Effects of GAD or RcsB box mutations on RcsB and GadE binding. (A) Point mutations introduced into the GAD and RcsB boxes. Conserved bases are shown in bold. (B) EMSA of gadA promoter fragment mutants in the presence of (His)6-RcsB (5 µM), and/or MBP-GadE (4 µM). NS: non-specific DNA.
