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. 2010 Feb 26;38(11):3546–3554. doi: 10.1093/nar/gkq097

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effects of RcsB or GAD box mutations on the transcriptional regulation of gadA in vivo. (A) β-Galactosidase activities of transcriptional gadA-lacZ fusions (either wild-type or M1 mutant promoter) with or without overexpressed (His)₆-GadE or (His)₆-RcsB. Strains carrying gadAp-lacZ and either pHGadE or pHRcsB plasmids with (white bars) or without (gray bars) 0.5 mM IPTG added for 1 h. (B) Same experiment as in A for gadAp_wt-lacZ and gadAp_M3-lacZ fusions with or without induction of RcsB synthesis. Activities were measured 30, 60 and 120 min after IPTG addition.