Table 1.
Bacterial strains and plasmids in this study
| Genotype | Source or reference | |
|---|---|---|
| Strains | ||
| UGM2 | MC4100 lon- (ΔPstI) | Lab. collection |
| MPC398 | MC4100 lon- ΔrcsCDB ΔrcsA::cat ΔgadE::kn | This study |
| MPC295 | trpDC::putPA1303-Km-gadA::lacZ (–165 to +788) | (9) |
| MPC297 | trpDC::putPA1303-Km-gadB::lacZ (–203 to + 788) | (9) |
| MPC299 | trpDC::putPA1303-Km-gadE::lacZ (–804 to +331) | (9) |
| CF6343 | MG1655 ΔlacIZ (MluI) | Lab. Collection |
| Plasmids | ||
| pHRcsB | pFab1, p15A-derived vector carrying ϕ(lacPUV5-his6-rcsB) | (20) |
| pHGadE | p15A-derived vector carrying ϕ(lacPUV5-his6-gadE) | This study |
| pMF533 | pMALc2E (New England Biolabs) containing malE-gadE fusion | (8) |
| pMRcsB | pMALc2E (New England Biolabs) containing malE-rcsB fusion | This study |
| pRS550 | ColE1 derived cloning vector, ApR, KnR | (17) |
| pRSgadA-lacZ | pRS550 containing gadAp fused to lacZ | This study |