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. 2010 Apr 5;38(11):e123. doi: 10.1093/nar/gkq192

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — (A) Schematic representation of the plasmids pShuttle-H6 and pBK2272-HPRT. The neomycin resistance marker genes (neo) are indicated as solid arrows. The Cre open reading frame is indicated as a shaded arrow. The PGK and the tk promoter are indicated as arrowheads. The HPRT selection marker gene is indicated as a striped arrow. The primer binding sites (horizontal arrows) used for genotyping and the sizes of the expected PCR products are indicated. (B) PCR analysis of DNA isolated from HEK 293 cells transfected with the indicated plasmids. The 2941-bp product is generated from the non-recombined pBK2272-HPRT plasmid. The 421-bp PCR product is indicative of a recombinase mediated cassette exchange between the PGKneo cassette (derived from plasmid pB2272-neo) and the PGK-HPRT cassette. Phage λ DNA digested with HindIII and EcoRI was used as molecular weight marker.