Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 5;38(11):e123. doi: 10.1093/nar/gkq192

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — PCR analysis using the primer combination bcas3, bcas10 and neoint.4 on DNA isolated from representative cell clones derived after electroporation of HM1 RMCE2272-98 cells with the plasmid pShuttle-G5 and selection of the transfected cells in medium containing 200 µg/ml of G418. The 1317-bp band is detected in all samples and represents the unmodified β-casein allele. Cell clones modified by an RMCE event, which has inserted the PGK-neo selection marker cassette, display an additional 1023-bp PCR product. Phage λ DNA digested with HindIII and EcoRI was used as molecular weight marker.