Skip to main content
. 2010 Apr 5;38(11):e123. doi: 10.1093/nar/gkq192

Table 1.

Primer combinations used for PCR analysis

Name Sequence Annealing temperature (°C) Amplicon length (bp)
bcas10 5′ GTA ACC ATA AAA CTT CTC CAG GGA CTT GG 3′
bcas3 5′ AGA GGA TCC GTA AGA CGT CAC CTG CTC ACC 3′ 55 1317
bcas10 5′ GTA ACC ATA AAA CTT CTC CAG GGA CTT GG 3′
bgalint.1 5′ TGT TGG TCA AAG TAA ACG ACA TGG TGA CT 3′ 55 1203
neoint.4 5′ GCG CAT CGC CTT CTA TCG CCT TCT TGA C 3′
bcas3 5′ AGA GGA TCC GTA AGA CGT CAC CTG CTC ACC 3′ 60 1023
PGK1 5′ CGA GGC CCG GCA TTC TGC ACG C 3′
CMVseq.1 5′ GGA CTT TCC AAA ATG TCG TA 3′ 50 2941
PGK5 5′ AAG CGC ATG CTC CAG ACT GCC TTG GGA AA 3′
CMVseq.1 5′ GGA CTT TCC AAA ATG TCG TA 3′ 51 421
PGK5 5′ AAG CGC ATG CTC CAG ACT GCC TTG GGA AA 3′
pBKpA 5′ GCT ATT GCT TTA TTT GTA ACC ATT A 3′ 52 622
hytk1 5′ AGA GCT GCA TCA GGT CGG AGA CGC TGT CG 3′
bcas3 5′ AGA GGA TCC GTA AGA CGT CAC CTG CTC ACC 3′ 57 1147
bcas6 5′ TAA GGG CCA GAG TAG ATC 3′
hytk2 5′ TCC TGG ATT ACG ACC AAT CG 3′ 48 215
bcas6 5′ TAA GGG CCA GAG TAG ATC 3′
PGK5 5′ AAG CGC ATG CTC CAG ACT GCC TTG GGA AA 3′ 50 936
neoint.4 5′ GCG CAT CGC CTT CTA TCG CCT TCT TGA C 3′
pBKpA 5′ GCT ATT GCT TTA TTT GTA ACC ATT A 3′ 51 1074
neoint.2 5′ CCA GTC ATA GCC GAA TAG CCT CTC CAC CC 3′
pBKpA2 5′ TTC ACT GCA TTC TAG TTG TGG TTT GTC 3′ 56 1106
DSred-1R 5′ AGC GCA TGA ACT CGG TGA TGA C 3′
CMVseq.1 5′ GGA CTT TCC AAA ATG TCG TA 3′ 59 529