Table 2.
Recombinase mediated cassette exchange efficiency is dependent on electroporation conditions
| Electroporation conditions | Number of colonies | Site-specific integration (%) |
|---|---|---|
| 250 V/960 μF (+Cre) | 221 ± 17 | 100 (12 of 12) |
| 250 V/960 μF (+Cre) | 73 ± 8 | 92 (11 of 12) |
| 250 V/960 μF (+Cre) | 154 ± 7 | 83 (10 of 12) |
| 250 V/960 μF (+Cre) | 68 ± 6 | 92 (11 of 12) |
| 800 V/3 μF (+Cre) | 53 ± 5 | 0 (0 of 12) |
| 800 V/3 μF (+Cre) | 21 ± 2 | 0 (0 of 12) |
| 800 V/3 μF (+Cre) | 46 ± 3 | 0 (0 of 12) |
| 800 V/3 μF (+Cre) | 61 ± 7 | 0 (0 of 12) |
| 800 V/3 μF (−Cre) | 57 ± 3 | nd |
| 800 V/3 μF (−Cre) | 62 ± 6 | nd |
Colony numbers are average values from four cell culture dishes (±SD). Site-specific integration was assessed by PCR as shown in Figure 1. nd: not determined.