Table 3.
Selection | pMC1-Cre | Colonies Gene Juice transfection (1.5 pg DNA per cell) | Colonies electroporation (2 pg DNA per cell) |
---|---|---|---|
G418 | (−) | 178, 131 | 78, 40 |
G418 | (+) | 42, 38 (3 of 17 positive, 18%) | 178, 155 (7 of 25 positive, 28%) |
G418/Ganc. | (−) | 133, 146 | 54, 78 |
G418/Ganc. | (+) | 71, 87 | 208, 218 |
pMC1-Cre was co-transfected with the plasmid pB2272-neo in a 3 : 1 ratio.