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. 2010 Apr 5;38(11):e123. doi: 10.1093/nar/gkq192

Table 3.

Recombinase mediated cassette exchange efficiency after liposome transfection and electroporation in two experiments conducted in parallel

Selection pMC1-Cre Colonies Gene Juice transfection (1.5 pg DNA per cell) Colonies electroporation (2 pg DNA per cell)
G418 (−) 178, 131 78, 40
G418 (+) 42, 38 (3 of 17 positive, 18%) 178, 155 (7 of 25 positive, 28%)
G418/Ganc. (−) 133, 146 54, 78
G418/Ganc. (+) 71, 87 208, 218

pMC1-Cre was co-transfected with the plasmid pB2272-neo in a 3 : 1 ratio.