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. Author manuscript; available in PMC: 2010 Jun 20.
Published in final edited form as: Anticancer Res. 2009 Dec;29(12):4959–4964.

Figure 2.

Figure 2

Effect of ATRA on histone acetylation and DNA methylation at the hTERT promoter and telomerase activity. (A) Chromatin immunoprecipitation. Chromatin complexes were precipitated with anti-H3-K9-Ac and resultant purified DNA was amplified by PCR with primers specific for the hTERT promoter. “Input” represents 1% of samples removed prior to the immunoprecipitation and amplified by hTERT primers. (B) Schematic of the 24 CpG sites in the hTERT promoter region represented by boxes. Underdetermined: methylation status could not be determined (which generally indicates partially methylated CpGs). (C) TRAP assay. The height and intensity of the ladder correlates with telomerase activity. IC: internal control.