Fig. 4.

5′-RACE analysis of the AL3 transcript. (a) Schematic representation of the AL3 ORF and primers used for 5′-RACE. K and M were used in PCR and nested PCR to map the 5′ end of AL3. (b) Total RNA were prepared from RS cells infected with dLAT2903R (R), dLAT2903 (2903), cells stably transfected with LAT (DC-LAT6; LAT6) or TG extracted from infected mice (Swiss Webster mice or C57BL/6 mice) for 31 days. 5′ RACE was performed as described in Methods. Lane M is a 100 bp DNA Ladder (Invitrogen). (c) Summary of AL3 5′-RACE results. An arrow denotes the AL3 5′ terminus mapped by 5′ RACE. AL3 RNA indicates the DNA sequence corresponding to the AL3 RNA sequence. Dashes in the LAT sequence indicate identity to the AL3 sequence. ATG sequences in bold type are in-frame methionine residues within the AL3 ORF. AL3-M is the primer sequence used in the 5′-RACE analysis. Images were prepared as described in Fig. 2.