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. 2010 Apr 21;4(7):1227–1239. doi: 10.1017/S1751731110000595

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Copyright © The Animal Consortium 2010 The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/2.5/>. The written permission of Cambridge University Press must be obtained for commercial re-use.

The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/2.5/>. The written permission of Cambridge University Press must be obtained for commercial re-use.

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Ovaries (hematoxylin-stained sections) of 18-month-old ewes exposed from conception to control inorganic fertiliser (a, b) or sewage sludge fertiliser (c, d); panels a and c ×10, b and d ×40 objective magnification. Treated ovaries have a higher proportion of unhealthy primordial and activating primordial follicles. Unhealthy follicles include follicles with intense nuclear staining (c, d), vacuoles (c) and clusters of two or more oocytes, which often share a common layer of granulosa cells (c, d).