Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Description* | Source (reference) |
|---|---|---|
| Pseudomonas aeruginosa | ||
| PAO-SW | Prototrophic PAO1 strain | S. E. H. West (West et al., 1994b) |
| PAOΔvfr | vfr deletion of PAO-SW | S. E. H. West (Albus et al., 1997) |
| PAOΔpvdS | pvdS isogenic mutant of PAO-SW; pvdS disruption; Gmr | This study |
| PAOΔregA | regA isogenic mutant of PAO-SW; regA internal deletion; Gmr | This study |
| Plasmids | ||
| pSW205 | lacZ translational fusion vector that replicates stably in P. aeruginosa; Cbr | S. E. H. West (Storey et al., 1990) |
| pSW228 | toxA–lacZ translational fusion in pSW205; Cbr | S. E. H. West (West et al., 1994a) |
| pRL88 | regA(P1/P2)–lacZ translational fusion in pSW205; Cbr | D. Storey (Storey et al., 1990) |
| pAM21-2 | pUC18 recombinant plasmid in which toxA is expressed from the lac promoter carried on pKT230; Cbr, Kmr | This study |
| pIN9 | 1.5 kb AvaI–PstI fragment carrying intact regA from PA103 plus 40 bp of the regA upstream region expressed from the lac promoter in pUC18; Cbr | This study |
| pIN10 | pIN9 carrying the 1.8 kb stability fragment for replication of plasmid in P. aeruginosa; Cbr | This study |
| pUCP19 | E. coli–P. aeruginosa shuttle vector; Cbr | H. P. Schweizer (Schweizer, 1991) |
| pKF917 | pUCP19 carrying intact vfr; Cbr | S. E. H. West (West et al., 1994b) |
| pVLT31 | Broad-host-range expression vector; Tcr | M. Vasil (Ochsner et al., 1996) |
| pPVD31 | pVLT31 recombinant plasmid in which pvdS is expressed from the tac promoter; Tcr | M. Vasil (Ochsner et al., 1996) |
| pMP220 | Broad-host-range lacZ transcriptional fusion vector; Tcr | P. Visca (Spaink et al., 1987) |
| pMP220 : : PpvdS | pvdS–lacZ transcriptional fusion in pMP220; Tcr | P. Visca (Ambrosi et al., 2002) |
| pMP190 | Broad-host-range lacZ transcriptional fusion vector; Cmr, Smr | I. Lamont (Spaink et al., 1987) |
| pMP190 : : PpvdE | pvdE–lacZ transcriptional fusion in pMP190; Cmr, Smr | P. Visca (Leoni et al., 2000) |
| pJQpvdS : : Gm | pvdS interrupted with a Gm cassette carried in the sacB suicide vector pJQ200SK; Gmr | D. Storey (Hunt et al., 2002) |
| pSUP203-ΔregA : : Gm | Mobilizable suicide plasmid carrying a Gm cartridge between regA-flanking regions; Gmr | M. Vasil (Ochsner et al., 1996) |
*Cb, Carbenicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Tc, tetracycline.