Fig. 3.

Analysis of rot gene expression in the different wild-type strains at various phases of growth. Northern blots were hybridized with 450 bp DNA fragments containing the coding region of the rot gene to detect the ∼0.6 knt rot transcript. Lanes 1–6, 10 μg cellular RNA per lane from the growing cultures at OD₆₀₀ 0.3, 0.7, 1.1, 1.4, 1.7 and overnight (stationary) (Fig. 1b). The region containing the 23S and 16S rRNA of the ethidium bromide-stained gel used for blotting is shown as a loading control. The third and sixth panels from the top represent Western blots of intracellular extracts from the different wild-type strains probed with anti-Rot polyclonal antibodies. Equivalent amounts of extracts (20 μg) from the different phases of growth (OD₆₀₀ ∼0.7, early exponential; OD₆₀₀ ∼1.1, exponential; OD₆₀₀ ∼1.7, post-exponential) were used to detect Rot expression.