Fig. 6.

Analysis of expression of the sarS gene in the different wild-type strains at various phases of growth. Northern blots were hybridized with 800 bp DNA fragments containing the coding region of the sarS gene to detect the 0.95 knt sarS major transcript. Lanes 1–6, 10 μg cellular RNA per lane from the growing cultures at OD₆₀₀ 0.3, 0.7, 1.1, 1.4, 1.7 and overnight (stationary) (Fig. 1b). The region containing the 23S and 16S rRNA of the ethidium bromide-stained gel used for blotting is shown as a loading control. The third and sixth panels from the top represent Western blots of intracellular extracts from the different wild-type strains probed with anti-SarS polyclonal antibodies. Equivalent amounts of extracts (20 μg) from the different phases of growth (OD₆₀₀ ∼0.7, early exponential; OD₆₀₀ ∼1.1, exponential; OD₆₀₀ ∼1.7, post-exponential) were used to detect SarS expression.