Fig. 7.

Northern blot analysis of the sarR (0.5 knt) and sarV (0.5 knt) transcripts in the different wild-type S. aureus strains. Blots were probed with 400 bp sarR and 360 bp sarV fragments containing the entire coding region of the sarR and sarV genes. A total of 10 μg cellular RNA was loaded into each lane. Lanes 1–6, total cellular RNA from the growing cultures at OD₆₀₀ 0.3, 0.7, 1.1, 1.4, 1.7 and overnight (stationary) (Fig. 1b). The region containing the 23S and 16S rRNA of the ethidium bromide-stained gel used for blotting is shown as a loading control.