Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description or genotype | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | F−φ80lacZ ΔM15 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 (lacZYA-argF) U169 | Laboratory strain collection |
| CAG48315 | λ− phtpg–lacZ ΔrpoH : : Kan zhf50 : : Tn10, pRB11 | Zhou et al. (1988) |
| MG1655 | K-12 | Arnaud Gutierrez |
| MG1655/pRB11 | MG1655 containing plasmid pRB11 expressing E. coli rpoH | This study |
| MG1655/pBADrpoH | MG1655 containing plasmid pBAD expressing F. tularensis rpoH | This study |
| MG1655ΔrpoH/pRB11 | MG1655/pRB11 transduced with P1 lysate from CAG48315 | This study |
| MG1655ΔrpoH/pBADrpoH | MG1655/pBADrpoH transduced with P1 lysate from CAG48315 | This study |
| F. tularensis strains | ||
| LVS | F. tularensis subspecies holarctica live vaccine strain | Laboratory strain collection |
| LVS/pFNLTP6gro-0851 | LVS with plasmid pFNLTP6gro_0851 expressing rpoH from the groES promoter | This study |
| LVS/pFNLTP6gro | LVS with plasmid pFNLTP6gro with no insert | This study |
| Plasmids | ||
| pRB11 | pBR322 derivative with E. coli rpoH gene downstream of IPTG-inducible tac promoter | Yura et al. (2007) |
| pBAD | araBAD promoter, araC, pBR322 origin, AmpR | Invitrogen |
| pBADrpoH | F. tularensis rpoH gene cloned downstream of arabinose-inducible promoter of pBAD | This study |
| pFNLTP6gro | E. coli–F. tularensis shuttle vector with groE operon promoter, KmR AmpR, constructed from pFNLTP6gro-gfp by deleting gfp gene | Meibom et al. (2009); Maier et al. (2006) |
| pFNLTP6gro-0851 | E. coli–F. tularensis shuttle vector with F. tularensis rpoH downstream of groE operon promoter, KmR AmpR | This study |