Fig. 2.

Effect of the bLPO system on the growth of S. mutans and S. gordonii. (a) S. mutans was inoculated first and grown aerobically (5 % CO₂) for 16 h at 37 °C on BHI agar with (+SCN⁻) or without 10 mM SCN⁻ and with (+bLPO) or without 20 μg bLPO per 8 μl of inoculum. S. gordonii was then inoculated next to the pioneer colonizer (with or without bLPO in the inoculum), and the plates were incubated for 16 h and then photographed. The columns labelled Sm and Sg are reference cultures grown in spatial isolation. (b) The same protocol was followed, except that S. gordonii was the pioneer colonizer and S. mutans was the competing colonizer. (c) The same protocol was followed as for (a) and (b), except that S. gordonii was both the pioneer colonizer and the competing colonizer.