Figure 2.

Amino acids Q118 and Q248 are essential for KLF8 activation of cyclin D1 gene promoter. (A) Schematic diagram of single point mutations generated in either KLF8 full length or ΔN100 context. (B and C) the cyclin D1 gene promoter reporter luciferase assays were performed similarly as described in Figure 1. Equal expression of the KLF8 constructs is shown in Supplemental Figure 1. *p < 0.05 compared to WT (B) or ΔN100 (C).