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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Cycle. 2010 Feb;9(3):601–611. doi: 10.4161/cc.9.3.10606

Figure 3.

Figure 3

Q118 and Q248 are two critical residues required for KLF8 activation of cyclin D1 gene promoter. (A) Schematic diagram of Q118N, Q248N and/or Q118N-Q248N mutants in the context of full length KLF8 (WT) or with disruption of the repression motif (PVDLS to AVALF). (B–E) NIH3T3 (B and C) or T80 (D and E) cells were co-transfected with the cyclin D1 gene promoter reporter and the indicated constructs. Luciferase assays were performed similarly as described in Figure 1. Equal expression of the KLF8 constructs is shown in Supplemental Figure 1. *p < 0.05 compared to WT (B and D), AVALF (C), or ΔN100 (E).