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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Cycle. 2010 Feb;9(3):601–611. doi: 10.4161/cc.9.3.10606

Figure 4.

Figure 4

KLF8 requires p300 and PCAF to activate the cyclin D1 promoter in a HAT dependent manner. (A) Mouse embryonic fibroblasts (MEF) null for p300 (p300−/−) were co-transfected with the cyclin D1 promoter reporter plus the empty vector, KLF8, Q118N-Q248N-KLF8 (mKLF8), p300, or p300ΔHAT (mp300) in the indicated combinations. Luciferase assays were performed and cyclin D1 gene promoter activity levels were normalized to levels in KLF8-transfected wild type MEF (WT) cells. (B and C) Similar experiments were performed using CBP (B) or PCAF (C) WT and mutant constructs and the corresponding null cells. *p < 0.05. Expression of the transfected co-activator constructs is shown in Supplemental Figure 2.