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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Cycle. 2010 Feb;9(3):601–611. doi: 10.4161/cc.9.3.10606

Figure 7.

Figure 7

The Q118 and Q248 residues are required for KLF8 to promote cyclin D1 protein expression and cell cycle progression. (A) NIH3T3 cells or T80 cells were transfected with empty vector, KLF8, the Q118N-Q248N mutant (mKLF8), or the AVALF-Q118N-Q248N mutant (AVALF-mKLF8). After 16 h lysates were collected and subjected to western blot analysis using anti-HA, anti-cyclin D1 and anti-actin antibodies. (B) NIH3T3 or T80 cells were transfected with empty vector, KLF8, or mKLF8. The G1 cell cycle progression of the cells was determined by measuring BrdU incorporation as described in Matherials and Methods. *p < 0.05.