Fig. 4.

Measurement of RI-10-, KI-10- and GL-9-specific CD8⁺ T cells in the PBMCs of AIV-infection-recovered donors and a healthy donor by tetramer staining. PBMCs of an HLA-A2⁺ healthy donor and three patients recovered from AIV infection (two HLA-A2⁺ AIV patients, named LXC and HJR, and an HLA-A2⁻ AIV patient) were stimulated with 10 μM RI-10, KI-10 or GL-9 in the presence of 20 U IL-2 ml⁻¹ for 1 week and the cells were harvested and stained using phycoerythrin (PE)-conjugated HLA-A*0201–RI-10, HLA-A*0201–KI-10 or HLA-A*0201–GL-9 tetramer, along with FITC-conjugated anti-CD8 monoclonal antibodies (mAbs) for cell staining. The numbers shown represent the percentage of tetramer⁺CD8⁺ cells within CD8⁺ T lymphocytes. The results are the representative of three independent experiments.