Fig. 3.

Promoter regions of crp target genes in Synechocystis. (a) Upstream regions of murF homologues in closely related cyanobacteria. CwatDRAFT_4119 and CY0110_02369 correspond to the indicated ORF identifiers in Crocosphaera watsonii and Cyanothece sp. CCY 0110, respectively. MSAs span conserved Crp sites to the conserved N-terminal amino acids belonging to protein homologues, thereby anchoring the alignment between two conserved regions. (b) Partial upstream region of chlA_II. (c) Upstream regions of sll1268 and slr0442. A secondary low-affinity site proposed by Omagari et al. (2008) is boxed with dashes. Proposed Crp binding sites identified by competitive gel shift in Fig. 1 are boxed. All putative transcriptional start sites were determined by RACE mapping in this work, are labelled (gene P_x), and are enclosed with small boxes. Suggested −10 sigma factor binding sites are underlined. Annotated translational start codons are double underlined. Tick marks are spaced 10.5 bases apart for reference. The murF P₁ is not shown, but described in the text.