Table 2.
Genes differentially expressed in osteoarthritis meniscal cells compared with normal control cells
| Gene name | Gene ID | Real-time RT-PCRa | Differential gene expressionb (fold) | Description | ||||
|---|---|---|---|---|---|---|---|---|
| OA1 | OA2 | OA3 | OA4 | OA5 | ||||
| ENPP1 | BF057080 | 2.1 | 1.7 | 2.0 | 2.8 | 1.9 | 2.0 | Ectonucleotide pyrophosphatase 1 |
| ANKH | AL833238 | 1.9 | 2.4 | 1.8 | 1.7 | 1.5 | 1.8 | Ankylosis, progressive homolog |
| MGP | NM_000900 | 5.3 | 11.8 | 4.7 | 21.6 | 0 | 17.6 | Matrix Gla protein |
| SRGN | NM_002727 | 3.3 | 2.2 | 3.1 | 8.3 | 3.1 | 1.9 | Serglycin |
aThe ratio of the relative expression level of a specific gene in osteoarthritis (OA) meniscal cells derived from five OA patients (RNA mixture) to the relative expression level of the specific gene in the control meniscal cells derived from three normal control subjects (RNA mixture), which were determined by real-time RT-PCR analysis with P < 0.01. bMicroarray analysis of the differential expression of a specific gene in the individual OA cells derived from five different OA patients compared with three normal control meniscal cells as a group. OA1, derived from a 65-year-old female OA patient; OA2, derived from a 56-year-old female OA patient; OA3, derived from a 50-year-old female OA patient; OA4, derived from a 52-year-old female OA patient; OA5, derived from a 61-year-old male OA patient; Normal 1, derived from a 36-year-old female osteosarcoma patient; Normal 2, derived from a 43-year-old female osteosarcoma patient; Normal 3, derived from a 12-year-old female osteosarcoma patient. Microarray analyses were carried out as described in Materials and methods. The raw microarray data can be found in the Gene Expression Omnibus [GEO:GSE19060] [49].