Table 2.
Oligonucleotide primers for site-directed mutagenesis
| Mutant | Primer (5’-3’) |
|---|---|
| S9A | GTTTCCAGCCTTTGGATACGCTGCGATCCATCGAATTCC |
| K12A | CCACGTGTTTCCAGCCGCTGGATACGATGC |
| T16A | CACCTGACCCACGCGTTTCCAGCCTTTGG |
| W17A | CAACATGCACCTGACCGCCGTGTTTCCAGC |
| K65A | CATCGGCCTTGAGGTGCGTCGCCACCAGCACCGGTTC |
| H67A | CACATCGGCCTTGAGGGCCGTCTTCACCAG |
| R90A | CATATCCCGCGGGTTCGCCACGAGATAGAG |
| S98A | GGCCATGCGATCGAGGCGAGCAGCATATC |
| R101A | TATCGAGGCCATGGCCATCGAGCTGAG |
| S106A | CTACGTCGTCGCGCGCTATCGAGGCCATGCG |
| R107A | GCTTTTTTCTACGTCGTCGGCCGATATCGAGGC |
| D108A | GCTTTTTTCTACGTCGGCGCGCGATATCGAGGCC |
| Y167A | GTGCTGACGATGCGTGCTGAGGACCTGAAGGGC |
| K171A | CGTTATGAGGACCTGGCGGGCGATCCGGTCGCACGG |
| E206A | GCTGCCTCCACGCTGGCGCGGATGCGTGAACTG |
| R207A | GCTGCCTCCACGCTGGAGGCGATGCGTGAACTG |
| E210A | GAGCGGATGCGTGCACTGGAGAAACGGAG |
| E212A | CGGATGCGTGAACTGGCGAAACGG |
| K213A | CGGATGCGTGAACTGGAGGCACGGAGCGAGCAGCAG |
| R214A | CGGATGCGTGAACTGGAGAAAGCGAGCGAGCAGCAG |
| M232A | GGTGATGCGAGAATGGCGAAAGGG |
| K233A | GATGCGAGAATGATGGCAGGGGG |
| G234A | GCGAGAATGATGAAAGCGGGACC |
| G235A | AGAATGATGAAAGGGGCACCTGGTG |
| P236A | ATGATGAAAGGGGGAGCTGGTGG |
| G238A | AAAGGGGGACCTGGTGCCGCGAGG |
| A239R | AAAGGGGGACCTGGTGGCCGGAGG |
| R240A | GGACCTGGTGGCGCGGCGCCCCAG |
| P241A | CCTGGTGGCGCGAGGGCCCAGTTC |
| Q242A | GGTGGCGCGAGGCCCGCGTTCGTG |
| R248A | CAGTTCGTGGGCGAGGGCGCGTACGACCAGTCCCTG |
| Q251A | GAGGGCAGGTACGACGCGTCCCTGTCCTTCTTG |