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Kinetic parameters for WT and G113N BMOH oxidation of methane and methanol
Error values represent sd from three replicates.
| Substrate | Km (μM) | kcat (s−1) | kcat/Km (10−4 μM−1 s−1) | |||
|---|---|---|---|---|---|---|
| WT | G113N | WT | G113N | WT | G113N | |
| Methane | 1100±140 | 340±20 | 1.3±0.1 | 0.13±0.01 | 11.8 | 3.8 |
| Methanol | 1250±60 | 750±40 | 1.6±0.1 | 0.21±0.02 | 12.8 | 2.8 |
