Skip to main content
. Author manuscript; available in PMC: 2010 Jun 21.
Published in final edited form as: J Biol Chem. 2005 Sep 28;280(48):40032–40040. doi: 10.1074/jbc.M506510200

FIGURE 4. Histological analysis of 8-day-old Pisd+/+ and Pisd−/− embryos.

FIGURE 4

Sagittal sections were made from embryos with surrounding deciduas. Tissues were fixed, embedded in paraffin, and serial sections were stained with hematoxylin/eosin. Size bars in A and C represent 500 nm, whereas for B and D size bars represent 200 nm. A and B, Pisd+/+ embryo. C and D, Pisd−/− embryo. A and C show overview of embryos with surrounding deciduas (Dc), placenta (P), and somites (S). B, detailed view of wild-type embryo that has developed the ectoderm (Ec), mesoderm (M), endoderm (En), and the amnion (Am). The extra-embryonic component is clearly defined. D, in Pisd-deficient embryos, no differentiation of the three germ layers was observed. The extra-embryonic component is absent, and blood cells (indicated by asterisks) directly surround the embryo.