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The major secretory protein recognized by VIG is VCP. (a, b) Concentrated supernatants from uninfected cells (Un) or cells infected with Dryvax (Dry) or LC16m8 (LC) were analysed by Western blot using VIG (a) or anti-VCP monoclonal antibody (b) as the primary antibody. The 28 and 30 kDa bands for Dry and LC16m8, respectively, are indicated by arrows.
