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CTCF interacts with the MH1 domain of Smad3, and exon 3 in Smad2 prohibits this interaction. Shown are GST pulldown assays of in vitro translated, [35S]methionine/cysteine-labeled FLAG-CTCF with the indicated GST-Smad3 deletion mutants (A) and GST-Smad2/Smad3 swapping mutants (B). The conditions and presentation are exactly as described for Fig. 2B. In B, M indicates the molecular size marker lane.
