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CTCF and Smads co-occupy CTCF-binding loci such as the H19 ICR. ChIP-Q-PCR analysis of four genomic fragments corresponding to three known imprinted loci, H19 ICR, KvDMR, and Xist, and a control intergenic region, clone 704. ChIP was performed in NMuMG cells stimulated with TGFβ1 for 4 h using specific antibodies against CTCF (A), Smad3 (B), and Smad4 (C). Q-PCR data show the average values and standard deviations calculated from triplicate determinations.
