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. 2010 Apr 23;285(26):19738–19746. doi: 10.1074/jbc.M110.101287

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Complementation of maturation of Nct and accumulation of Pen-2 by expression of TM8mt and TM9mt in DKO cells. A, immunoblot analysis of DKO cells stably transfected with WT or mutant PS1 (as indicated above the panel). Cell lysates were analyzed by immunoblotting with each antibody (as indicated below the panel). FL, full length; mNct, mature nicastrin; imNct, immature nicastrin. B, immunoblot analysis of the generation of NICD from NotchΔE-stable DKO cells. C, sandwich ELISA analysis of secreted Aβ from APP_NL-stable DKO cells transiently expressing TMD-swap PS1 mutants. The levels of Aβ40 and Aβ42 are shown by white and black bars, respectively. Error bars indicate S.E. D, co-immunoprecipitation analysis of 1% CHAPSO-solubilized fractions prepared from DKO cells transiently transfected with WT or mutant PS1. Soluble fractions were precipitated (IPed) by MAB5232 and then analyzed by immunoblotting with each antibody as indicated below the panel.