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. 2010 Apr 23;285(26):19738–19746. doi: 10.1074/jbc.M110.101287

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Stability of the HMW complex harboring TMD-swap PS1 mutants. A, analysis of stability of the HMW complex containing WT PS1 in DKO cells incubated in culture medium containing cycloheximide (CHX) (30 mg/ml). DKO cells after various incubation periods (0–12 h) were solubilized in BN-PAGE lysis buffer and analyzed by BN-PAGE analysis with each antibody (as indicated below the panel). In immunoblot using anti-Nct antibody, numbers shown above or below each lane denote the incubation period and the relative band intensity, respectively. Note that all components were stable over 12 h. B, analysis of stability of the HMW complex harboring TMD-swap PS1 mutant probed by anti-Nct antibody.