FIGURE 7.
Photoaffinity labeling experiments using TMD-swap PS1 mutants. A, CHAPSO-solubilized DKO cell membranes (DKO cells expressing PS1 holoproteins) were photoactivated in the absence (−) or presence (+) of the parent compound as a competitor and analyzed by immunoblotting with anti-PS1 NTF antibody (PS1NT). PS1 FL, full-length PS1. B, CHAPSO-solubilized DKO cell membranes (DKO cells co-expressing PS1 NTF and PS1 CTF individually from separate vectors) were photoactivated in the absence (−) or presence (+) of the parent compound as a competitor and analyzed by immunoblotting with anti-PS1NT antibody. Probes used are indicated at left of the panel.