FIGURE 6.

Chaperone activity assays of HspL and its variants (HspLF98A and HspLG118A) using GST-VirB8 as substrate. Thermal aggregation protection assays using GST-VirB8 (600 nm) was carried out at 50 °C in the absence (○) or presence of HspL-His₆ (▴), HspLF98A-His₆ (●), and HspLG118A-His₆ (■) at concentrations of 1.2 μm, respectively. GST (▾) alone incubated under the same conditions was used as a negative control. Aggregation was monitored in absorbance at 360 nm and presented as a function of time.