Skip to main content
. 2010 Apr 20;285(26):19767–19775. doi: 10.1074/jbc.M110.114520

FIGURE 4.

FIGURE 4.

Functional characterization of AdoMetT1. A, fluorescence microscopy analysis of LV39SNF4000.4 cells expressing AdoMetT1-GFP. LV39SNF4000.4 cells were transfected with a plasmid where the AdoMetT1 gene and the GFP gene were fused in-frame. Analysis of transfectants by fluorescence microscopy indicated that the fusion protein was localized mainly in the plasma membrane of the parasite. B, transport of 60 nm S-[3H]adenosylmethionine in L. major cells. Δ, L. major Friedlin; ■, L. major Friedlin transfected with AdoMetT1; □, LV39SNF4000.4; ▿, LV39SNF4000.4 transfected with AdoMetT1-GFP; ▾, LV39SNF4000.4 transfected with AdoMetT1. C, transport of 60 nm S-[3H]adenosylmethionine in L. tarentolae cells. ○, TarMTX1000.6 transfected with GFP; ♦, TarMTX1000.6 transfected with AdoMetT1-GFP; ◆, TarMTX1000.6 transfected with FT1; ▴, TarSNF.8; ▾, TarSNF.8 transfected with AdoMetT1-GFP. D, transport of 115 nm [3H]folic acid in L. tarentolae cells. ♦, TarMTX1000.6 transfected with AdoMetT1-GFP; ◆, TarMTX1000.6 transfected with FT1-GFP. The average of triplicate experiments are shown (B–D).