FIGURE 1.
The activation status of NPM-ALK is important for its binding to SHP1. Co-immunoprecipitation (IP) experiments using GP293 cells co-transfected with NPM-ALK (or its mutants) and SHP1 revealed binding of SHP1 to NPM-ALK (lane 2), but not the enzymatically inactive NPM-ALKK210R mutant (lane 3) or the NPM-ALKFFF mutant (lane 4). Immunoblotting (IB) with anti-SHP1 revealed a relatively equal amount of immunoprecipitated SHP1 proteins. Negative control reactions (−) were performed by omitting the use of anti-SHP1 antibody. Cells co-transfected with SHP1 and an empty vector (i.e. pcDNA3) were used as a negative control. Results shown are representative of three independent experiments.