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SpARC4 is a GPI-anchored protein. HEK cells expressing SpARC4 Myc were treated with (+) or without (−) PI-PLC, and the presence of SpARC4 was determined in media samples by Western blot analysis (A) and on the cell surface by immunocytochemistry (B). Scale bar, 50 μm. C, Western blot analysis of media samples from HEK cells expressing SpARC4 Myc or Myc-tagged SpARC4 lacking the C-terminal 17 amino acids (SpARC4 ΔC Myc).
