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. 2010 Apr 12;285(26):19900–19909. doi: 10.1074/jbc.M110.105312

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Lack of effect of the acidic-basic cluster on SpARC4 activity. A, Western blot analysis of Xenopus embryos (left) or HEK cells (right) expressing Myc-tagged SpARC4 lacking either the N-terminal 17 amino acids (SpARC4 ΔN Myc) or in which arginines 52 and 53 were mutated to alanine (SpARC4^R52A/R53A Myc). SpARC4 Myc was analyzed in parallel. B, immunocytochemical localization of SpARC4 ΔN Myc and SpARC4^R52A/R53A Myc in HEK cells. Scale bar, 5 μm. C, time courses for production of cADPR and NAADP by SpARC4 Myc, SpARC4 ΔN Myc, and SpARC4^R52A/R53A Myc. D, Western blot analysis of SpARC4 Myc and SpARC4^R52A/R53A. Arrowhead marks the expected migration of the putative cleavage product. Blots were deliberately overexposed.