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. 2010 Apr 12;285(26):19900–19909. doi: 10.1074/jbc.M110.105312

FIGURE 6.

FIGURE 6.

Regulation of SpARC4 activity by noncanonical active site residue. A, multiple sequence alignment of ADP-ribosyl cyclases showing lack of conservation of an active site tyrosine (asterisk) in SpARC4 and Schistosoma ADP-ribosyl cyclase. Abbreviations used are as follows: Ac, Aplysia californica; Hs, Homo sapiens; Sm, Schistosoma mansoni; Sj, Schistosoma japonica. B, Western blot analysis of Xenopus embryos (left) or HEK cells (right) expressing SpARC4 Myc or Myc-tagged SpARC4 in which tyrosine 142 was mutated to either tryptophan (SpARC4Y142W Myc) or histidine (SpARC4Y142H Myc). C, immunocytochemical localization of SpARC4Y142W Myc and SpARC4Y142H Myc in HEK cells. Scale bar, 5 μm. D, time courses for production of cADPR and NAADP by SpARC4 Myc, SpARC4Y142W Myc, and SpARC4Y142H Myc.