FIGURE 6.

Neuroprotection of bis(propyl)-cognitin in vitro. A, CGNs, at 8 days in vitro, were preincubated with bis(propyl)-cognitin (B3C) at different concentrations as indicated for 2 h and then exposed to 75 μm glutamate for another 24 h. Cell viability is measured using an MTT assay. All of the data, expressed as percentage of untreated control, are the means ± S.E. (error bars) of three separate experiments. Inset, plots show dose-response relationships for bis(propyl)-cognitin and memantine protection. B, CGNs, at 8 days in vitro, were preincubated with 3 μm bis(propyl)-cognitin (B3C) or bis(heptyl)-cognitin (B7C) for 2 h and then exposed to different concentrations of glutamate as indicated for another 24 h. The cell survival after the treatment with glutamate or glutamate following bis(propyl)-cognitin or bis(heptyl)-cognitin preincubation is expressed as percentage of untreated control.