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. 2010 Apr 13;285(26):19959–19966. doi: 10.1074/jbc.M110.101030

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Comparison of binary TF·FVIIa and ternary TF·FVIIa·FXa PAR2 signaling in HaCaT keratinocytes. A, TF·FVIIa-mediated ERK1/2 phosphorylation was determined after stimulation of HaCaT cells for 10 min with the indicated concentrations of wt FVIIa or T99Y (mean ± S.D., n = 4). B, ternary complex signaling was evaluated by measuring phospho-ERK1/2 levels (left panel) after 10 min of stimulation with 100 nm FX and 0.5 nm FVIIa. At this concentration FVIIa-TF binary signaling did not contribute to PAR2 activation. The amount of FXa generated at this time (right panel) was measured by chromogenic assay (mean ± S.D.; n = 5). The signaling induced by 5 nm FXa is included for comparison (light-colored bar).