FIGURE 2.

Surface plasmon resonance kinetic data and residuals for M158C hPRL (top), N152C hGH (middle), and N152C hPL (bottom) binding to the extracellular domain of the hPRL receptor in the presence of Zn²⁺. Each of the three lactogens was covalently attached to a CM-5 SPR chip; the fourth lane was activated but not populated with protein. Increasing concentrations of hPRLr (10 nm, 50 nm, 100 nm, 500 nm, 1 μm, 5 μm, 10 μm, 50 μm, and 100 μm) were flowed over the chip surface for 300 s to follow hormone/hPRLr binding. Subsequently, buffer was flowed over the chip surface, and hormone/hPRLr dissociation followed. Residues from the model fit to the data are presented beneath the binding data.