FIGURE 2.

The activity of the HOG pathway influences the sensitivity of yeast cells to tunicamycin. Cells of the wild-type (wt) W303-1A and corresponding mutant strains lacking the MAPK Hog1p (hog1Δ) and/or the protein phosphatases Ptp2p and Ptp3p, ptp2Δ, ptp3Δ, ptp2Δptp3Δ, hog1Δptp2Δ, and hog1Δptp2Δptp3Δ were assayed for growth in the presence of tunicamycin (Tn). All strains (supplemental Table S1) were made isogenic for auxotrophic markers by transforming with the integrative plasmids described by Gietz and Sugino (35), YIplac204 (TRP1), YIplac211 (URA3), and/or YIplac128 (LEU2). As a control, growth was inspected on YPD plates lacking tunicamycin or containing DMSO alone. Cells were pregrown, diluted, plated, and incubated as described in Fig. 1. A representative experiment is shown.