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. 2010 Apr 20;285(26):20097–20108. doi: 10.1074/jbc.M109.090092

FIGURE 5.

FIGURE 5.

Deletion of ozmM and complementation of the ozmM mutant with either intact ozmM or ozmM-AT1 (S81G) or ozmM-AT2 (S402G) mutant and their effect on OZM biosynthesis. A, schematic representation of constructs for the generation of the ZH9 deletion mutant strain and its genetic complementation strains ZH10, ZH14, and ZH15. Details of site-specific mutagenesis with OzmM-AT1 and OzmM-AT2 are shown. B, HPLC analysis of OZM production in wild-type (I), ZH9 mutant (II), ZH 10 mutant (III), ZH14 mutant (IV), and ZH15 mutant (V) strains. ♦, OZM.