TABLE 1.
Deduced ORF functions in the ozm biosynthetic gene cluster
| Gene | Sizea | Protein homologb | Proposed function |
|---|---|---|---|
| aa | |||
| orf(−5) | 335 | Franean1_1604(ABW11043, 52/39) | Transcriptional regulator |
| orf(−4) | 775 | SAV_1033(NP_822208 83/71) | Integral membrane protein |
| orf(−3) | 158 | SAV_1074(NP_822249, 83/79) | Bacterioferritin comigratory protein |
| orf(−2) | 339 | SACE_5658(CAM04844, 59/47)) | Transcriptional regulator |
| orf(−1) | 315 | CMS_2856(YP_001711489, 84/71) | Nucleoside hydrolase |
| Upstream boundary of ozm cluster | |||
| ozmA | 482 | SgcB(AAF13999, 28/43) | Antibiotic efflux protein |
| ozmB | 366 | GdmH(ABI93783, 65/76) | Glyceryltransferase/phosphatase |
| ozmC | 325 | DpsC(AAA65208, 25/39) | Acyltransferase |
| ozmD | 366 | GdmI(ABI93784, 64/75) | Acyl-dehydrogenase |
| ozmE | 93 | GdmJ(ABI93785, 63/78) | ACP |
| ozmF | 221 | TtmC(AAZ08058, 60/77) | O-Methyltransferase |
| ozmG | 287 | TtmB(AAZ08059, 63/72) | 3-Hydroxyacyl-CoA-dehydrogenase |
| ozmH | 7737 | PksP(E69679, 35/51) | Hybrid NRPS/PKS |
| ozmJ | 2926 | ObsC(AAS00421, 52/64) | PKS |
| ozmK | 1202 | BryB(ABK51300, 34/50) | PKS |
| ozmL | 1993 | McyA(AAF00960, 37/55) | NRPS |
| ozmM | 1039 | MmpIII(AAM12912, 47/59) | Acyltransferase/oxidoreductase |
| ozmN | 4971 | LnmJ(AF484556, 38/48) | PKS |
| ozmO | 1196 | PedF(AAS47564, 42/56) | NRPS |
| ozmP | 382 | - | Unknown |
| ozmQ | 842 | NosB(AAF15892, 55/69) | PKS |
| ozmR | 308 | Orf5(BAA32133, 53/64) | Transcriptional regulator |
| ozmS | 214 | SC5F8.18(CAB93746, 71/78) | Transporter |
| ozmT | 439 | SCH63.25(CAC10316, 77/83) | Thr-tRNA synthetase |
| ozmU | 929 | AfsR(BAA14186, 33/45) | Transcriptional activator |
| Downstream boundary of ozm cluster | |||
| orf(+1) | 445 | CypC (ABS73471, 42/59) | Cytochrome P450 |
| orf(+2) | 221 | Unknown | |
| orf(+3) | 282 | SC5F8.24 (CAB93752, 70/81) | RNA polymerase sigma factor |
| orf(+4) | 344 | Unknown | |
| orf(+5) | 700 | Unknown | |
a Sizes are in amino acids (aa).
b Given in parentheses are accession numbers and percentage identity/percentage similarity.