FIGURE 5.

Tti1 and Tel2 interact with and stabilize PIKKs. A, HEK293T cells were transfected with empty vector, FLAG-Tti1, or FLAG-Tel2. Two days later, cells were lysed, and immunoprecipitation was performed with anti-FLAG antibody. The lysate lanes contain one-twenty fourth of the lysate volume used for immunoprecipitation. B, HeLa cells were treated with siRNAs against Tti1 or/and Tel2 for 4 or 6 days. Transfection was performed every other day. Cells were then harvested, and immunoblot analysis was performed with the indicated antibodies.