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. 2010 Apr 23;285(26):20128–20136. doi: 10.1074/jbc.M109.099101

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FIGURE 5. — NTD physically associates with TICAM-1-TIR. HEK293FT cells were transfected with FLAG-tagged NTD and HA-tagged TICAM-1-N (1–359 aa), TIR domain (387–566 aa), or TICAM-1-C (567–712 aa). After 24 h, cells were lysed, and NTD was immunoprecipitated (IP) using an anti-FLAG mAb. The immunoprecipitants were resolved on SDS-PAGE (12.5% gel) under reducing conditions followed by immunoblotting (IB) with anti-HA pAb or anti-FLAG mAb. Whole cell lysates (WCL) were subjected to immunoblotting with anti-HA pAb to detect protein expression. Open arrowheads indicate immunoglobulin light chain (Ig LC). Molecular weight markers are on the right.