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. 2010 Apr 23;285(26):20128–20136. doi: 10.1074/jbc.M109.099101

FIGURE 7.

FIGURE 7.

Δ180 mutant had high potential to activate the IFN-β promoter compared with wild-type TICAM-1. A, IFN-β promoter activation by the Δ180 mutant. HEK293 cells were transfected with empty vector or expression plasmid for wild-type (WT) TICAM-1 or Δ180 mutant (0.1 and 1.0 ng) together with the IFN-β promoter reporter. Luciferase activity was measured 24 h after transfection. Representative data from a minimum of four separate experiments, each performed in triplicate, are shown. B, protein expression of wild-type and the Δ180 mutant in HEK293 cell lysates. IB, immunoblot. C, confocal images of HeLa cells expressing a low level of HA-tagged wild-type TICAM-1 (upper panels) or Δ180 mutant (lower panels). Cells were transfected with the expression plasmid for wild-type TICAM-1 or Δ180 mutant (0.1 ng). After 24 h, cells were stimulated with buffer alone or 10 μg/ml poly(I-C) for 30 min, and fixed cells were labeled with anti-HA pAb and Alexa Fluor 568-labeled secondary Ab. The Δ180 mutant formed a speckle-like signalosome in unstimulated cells. Red, wild-type and Δ180 mutant; blue, DAPI-stained nuclei. Bar, 10 μm.