FIGURE 5.

Endogenous Rac1 activity promotes recruitment of CD2AP to cell-cell contacts. A, HeLa cells transfected with Myc-Rac1-Q61L were stained for endogenous CD2AP. For the indicated proteins, intensity profiles across cell-cell contacts and in the direction indicated by the arrows, were generated using Zeiss Zen software. B and C, HeLa cells transfected with CD2AP-GFP were either treated with 300 ng/ml of CNF1 for 1.5 h or with 100 μm 8-CPT-cAMP for 2 h. Cells were co-stained for cell-cell contacts using either β-catenin or F-actin as a marker. Scale bars, 10 μm.